Lexogen Newsletter (RNA Salon partner)

The success of any RNA-Seq project begins with thoughtful planning. Choosing the right sequencing method and ensuring solid experimental design are key to generating meaningful, reproducible results. Here’s a quick guide to help you navigate those decisions.   WTS vs. 3′ mRNA-Seq: Choose with Confidence Whole Transcriptome Sequencing (WTS) gives a complete RNA view, capturing isoforms, splicing, non-coding RNAs, and complex transcript structures. It’s ideal for deep mechanistic insights. 3′ mRNA-Seq is ideal for streamlined, cost-efficient gene expression quantification. It requires lower sequencing depth, simplified data analysis, and works exceptionally well with degraded or FFPE samples. Despite differences, both methods deliver highly comparable pathway-level conclusions — making the right choice a matter of scope, sample type, and budget.   Want more comparisons and case studies? Check our blog for deeper insights →     🎥 From Design to Data: Key Webinar Takeaways Beyond the Reads: Designing Successful RNA-Seq Experiments and Data Analyses Our “Beyond the Reads” webinar walks through real datasets to show how design decisions influence results: Choosing between WTS and 3′ mRNA-Seq Live data analysis with Omics Playground Why controls and replicates ensure biological relevance   Watch the full webinar →     🎥 Extra Resource – Your Free RNA-Seq Guide (Lexicon E-Book) New to RNA-Seq or refining your workflow? Lexogen’s LEXICON E-Book is your educational companion, covering: Experimental planning Sequencing & analysis strategies Tips for FFPE, liquid biopsies & challenging samples   Download LEXICON for free →     🚀 Ready to elevate your research?   At Lexogen, our top-tier NGS solutions are designed to fuel your research projects. Explore how our products can meet your needs and help you achieve your goals. We're committed to supporting you during every step of the way: from helping to plan your experiments to analyzing your data! Learn more about our products >>>   We 💚 RNA Visit our website www.lexogen.com   Lexogen GmbH, Campus Vienna Biocenter 5, Vienna, Austria 1030, +43 (0) 1 345 1212 Unsubscribe Manage preferences